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Свидетельство о регистрации ПИ №ФС77-235В7
от 8 марта 2008 г. ISSN: 1815-445X
от 8 марта 2008 г. ISSN: 1815-445X
Different bone marrow cells autotransplantation influence on morphofunctional state of myocardium after experimental infarction
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PDF1. Saint-Petersburg I.P. Pavlov State Medical University
2. Institute of Cytology, RAS, Saint-Petersburg
3. Kirov Military Medical Academy, Saint-Petersburg
2. Institute of Cytology, RAS, Saint-Petersburg
3. Kirov Military Medical Academy, Saint-Petersburg
Keywords:
myocardial infarction, multipotent mesenchymal stromal cells, nucleated cells, bone marrow, autologous transplantation, electrocardiography, echocardiography.
myocardial infarction, multipotent mesenchymal stromal cells, nucleated cells, bone marrow, autologous transplantation, electrocardiography, echocardiography.
The contribution aims to compare influence of intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells and bone marrow nucleated cells on a myocardium morphofunctional state after infarction. Chinchilla rabbits were exposed in the experiment. Myocardial infarction was modeled by ligating the anterior descending branch of the left coronary artery. The condition was evaluated with functional (electrocardiography, echocardiography] and morphologic methods. There were three groups of animals. To the first group animals (control, n = 13] a-MEM growth medium was injected into the damaged area; a culture of bone marrow multipotent mesenchymal stromal cells (2x10s] was introduced to the 2nd group animals (n = 14], while the animals of the 3rd group (n = 14] being given bone marrow nucleated cells (2x10е]. It has been shown that intramyocardial autotransplantation of bone marrow multipotent mesenchymal stromal cells in experimental myocardial infarction resulted in ischemic area restriction, normalization of systolic function indices as well as stimulation of angiogenesis. At the same time intramyocardial autotransplantation of bone marrow nucleated cells was followed by an expansion of ischemic area and reduction of systolic function indices as compared with the controls, although angiogenesis activation occurred.
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