cellTranspl.ru - Gene expression under control of regulatory elements of thermal shock gene of drozophyla in transgenic larvae germ of loach Misgurnus fossilis L.

Gene expression under control of regulatory elements of thermal shock gene of drozophyla in transgenic larvae germ of loach Misgurnus fossilis L.

HTML,

PDF, Volume I, №4, 2006

L.E. Andreeva, N.V. Khaydarova, A.V. RodrigesBlanko, N.N. Kananaykina, A.V. Revishchin, V.Z. TarantuP, L.I. Korochki.n. г, G.V. Pavlova

Keywords:
gene expression, thermal shock proteins

Rating: 0.00 Rate article: 1 2 3 4 5 Views: 1623, comments: 0

The efficiency of transient expression of lacZ and gfp genes-reporters under control of two different promoter elements of drozophyla hsp70 thermal shochk gene in transgeneic larva of Fogeneration loach Misgurnus fossilis L. were studied. The expression of genes in early larva and in the prelarval stage was mosaic and depended upon the amount of genetic material introduced. At the same time gene expression did not depend upon external tempreture increase, i.e. it appeared without thermal shock. In 2-3 day larva the efficiency of transient expression was maximal and reached 100% in separate experimental series. Thus, regulatory elements of drozophula thermal shock gene are capable to provide constitutional expression of gene-reporter in loach larva.

1. .Westerfield M., Wegner J., Jegalian B.G. et al. Specific activation of mammalian Hox promoters in mosaic transgenic zebrafish. Genes Dev. 1992; 6: 591-8
2. Андреева Л.Е., Григоренко А.П., Гордеева О.Ф., Дворянчиков ГА. Экспрессия CMV-lacZ и RSV-lacZ-генов в трансгенных эмбрионах рыб и мышей. Генетика 1996; 3:1661 -8
3. Molina A., Biemar F., Muller F. et al. Cloning and expression analysis of an inducible HSP70 gene from tilapia fish. FEBS Lett 2000; 474: 5-10
4. Zeng Z., Liu X., Seebah S., Gong Z. Faithful expression of living color reporter genes in transgenic medaka under two tissue-specific zebrafish promoters. Dev. Dyn. 2005; 234: 387-92
5. Козлов А.П., Решетников В.Л., Корж В.П., Нейфах А.А. Чужеродная ДНК в развивающихся зародышах вьюна Misgumus fossilis L Мол. биология 1988; 22:1614-22
6. Колесников В.А., Алимов А.А., Барминцев В.А. и др. Высокоскоростная механическая инъекция чужеродной ДНК в яйцеклетки рыбы. Генетика 1990; 26:2122-6
7. Андреева Л.Е., Дворянчиков ГА. Анализ экспрессии RSV-lacZ-гена в трансгеннных эмбрионах вьюна Misgurnus fossilis L при различных вариантах инъекций. Генетика 1995; 31: 759-66
8. Krone P.H., Evans T.G., Blechinger S.R. Heat shock gene expression and function during zebrafish embryogenesis. Semin. Cell Dev. Biol. 2003; 14: 267-74
9. Krone P.H., Heikkila J.J. Expression of microinjected hsp 70/CAT and hsp 30/CAT chimeric genes in developing Xenopus laevis embryos. Dev. 1989; 106: 271-81
10. Cadoret J.P., Boulo V., Gendreau S., Mialhe E. Promoters from Drosophila heat shock protein and cytomegalovirus drive transient expression of luciferase introduced by particle bombardment into embryos of the oyster Crassostrea gigas. J. Biotechnol. 1997; 56:183-9
11. Saraiva E., Fampa P., Cedeno V. et al. Expression of heterologous promoters in Lutzomyia longipalpis and Phlebotomus papatasi [Diptera: Psychodidae) cell lines. J. Med. Entomol. 2000; 37: 802-6
12. Adam A., Bartfai R, Lele Z. et al. Heat-inducible expression of a reporter gene detected by transient assay in zebrafish. Exp. Cell Res. 2000; 256: 282-90
13. Smith R.C., Machluf M., Bromley P. et al. Spatial and temporal control of transgene expression through ultrasound-mediated induction of the heat shock protein 70B promoter in vivo. Hum. Gene Ther. 2002; 13: 697-706
14. Павлова Г.В., Мануйлова E.C., Арсеньева Е.Л. й др., Экспрессия белка BFP в трансфецйрованных эмбриональных стволовых клетках. Клеточные технологий в 6йологйй й медицине 2005; 2: 99-102.
15. Нейфах А.А. Использование метода радиационной йнэктйвэцйй ядер для исследования их функций в раннем развитии рыб. Журн. Общ. Биологии 1959:20:202-13
16. Amin J., Mestril R., Schiller P. et al. Organization of the Drosophila melanogaster hsp70 heat shock regulation unit. Mol. Cell Biol. 1987; 7:1055-62
17. Андреева Л.Е., Жаданов А.Б. Кузнецов Ю.М. Экспрессия гена |3-галактозйдазы в трансгенных эмбрионах вьюна Misgurnus fossilis L Генетика 1993:29:740-7
18. Thorey I.S., Meneses J.J., Neznanov N. et al. Embryonic expression of human keratin 18 and K18-beta-galactosidase fusion genes in transgenic mice. Dev. Biol. 1993; 160: 519-34
19. Amin J., Mestril R., Lawson R. et al. The heat shock consensus sequence is not sufficient for hsp70 gene expression in Drosophila melanogaster. Mol. Cell Biol. 1985; 5:1 97-203.
20. Takada Т., Iida K., Awaji T. et al. Selective production of transgenic mice using green fluorescent protein as a marker. Nat. Biotechnol. 1997; 15:458-61
21. Conway G., Torrejon M., Lin S., Reinsch S. Fluorescent tagged analysis of neural gene function using mosaics in zebrafish and Xenopus laevis. Brain Res. 2006; 1070: 150-9
22. Дворянчиков Г.А., Серова И.А., Андреева Л.Е. й др. Секреция биологически активного гранулоцйт колонйе-стймулйрующего фактора [Г- КСФ) человека в молоке трансгенных мышей. Генетика 2005; 41:1330-7
23. Stuart G.W., Vielkind J.R., McMurray J.V., Westerfield M. Stable lines of transgenic zebrafish exhibit reproducible patterns of transgene expression. Dev. 1990:109:577-84
24. Halloran M.C., Sato-Maeda M., Warren J.T. et al. Laser-induced gene expression in specific cells of transgenic zebrafish. Dev. 2000; 127:1953-60