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Registration number: ПИ №ФС77-235В7,
8th March 2008. ISSN: 1815-445X
8th March 2008. ISSN: 1815-445X
Searching for Suitable Conditions for Proliferation and Differentiation of Stem and Progenitor Cells Isolated from CNS of Human and Animal Brain
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PDF1. Scientific Research Institute for Epidemiology and Microbiology, Minsk, Belarus
2. Belarusian State Medical University, Minsk, Belarus
2. Belarusian State Medical University, Minsk, Belarus
Keywords:
neural stem and progenitor cells, morphology, phenotype, proliferation, differentiation
neural stem and progenitor cells, morphology, phenotype, proliferation, differentiation
The technology for preparation of cultures of stem and progenitor cells (SPC]
from neurogenic zones of human and animal brain was developed. Optimum medium
composition for proliferation of neural SPC forming neurospheres in suspension
culture was determined. Characterization of morphology and phenotype of
cultured cells with use of monoclonal antibodies for markers of stem,
progenitor and mature cells was performed. The ability of SPC to grow and
accumulate biomass during three passages was established. Comparative
evaluation of growth intensity of neurospheres isolated from different regions
of human and animal brain was pursued. More intensive proliferation of SPC
isolated from animal subependymal zone of brain ventricles and olfactory bulb
was recognized.
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