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Registration number: ПИ №ФС77-235В7,
8th March 2008. ISSN: 1815-445X
8th March 2008. ISSN: 1815-445X
The comparative cytogenetic analysis multipotent mesenchymal stromal cells of early passages and lymphocytes the person
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PDF1. The St.-Petersburg State University, Russia
2. GU Scientific research institute of Obstetrics and Gynecology of D.O. Otta RAMS, St.-Petersburg, Russia
3. Trans-Technologies Ltd., Saint-Petersburg, Russia
2. GU Scientific research institute of Obstetrics and Gynecology of D.O. Otta RAMS, St.-Petersburg, Russia
3. Trans-Technologies Ltd., Saint-Petersburg, Russia
Keywords:
multipotent mesenchymal stromal cells, karyotyping, chromosomal aberrations
multipotent mesenchymal stromal cells, karyotyping, chromosomal aberrations
We have analyzed karyotype of early-passaged human mesenchymal stem cells
(hMSC) compared to that of peripheral blood lymphocytes from the same
individuals. Karyotyping was performed on cultures of hMSC and PHA-stimulated
peripheral blood lymphocytes donated by 8 healthy men and women volunteers.
Cytogenetic analysis of hMSC cultures after each passage, from fourth to
seventh, revealed no metaphase plates with abnormal chromosome number and/or
structure. In all cases we established normal karyotype, corresponding
karyotype of PHA-stimulated lymphocytes from the same individuals. Thus, in
early-passaged cultures of hMSC from donors with normal karyotype in
lymphocytes, no cells with chromosomal and/or genomic mutations were detected.
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