Modeling of functional osteogenesis using biodegradable matrix and autogenic stromal cells of subcutaneous adipose tissue
V.S. Melikhova1, I.N. Saburina1, A.A. Orlov2,I.A. Martirosyan2, V.S. Repin1, N.I. Novikova3, A.N. Murashev3
1. Institute of General Pathology and Pathophysiology, Moscow
2. Clinical Hospital № 85, Dentistry Clinics Dental «Academy»
3. Institute of Bioorganic Chemistry (Branch] RAS, Moscow
Keywords:
stem cells, bone tissue engineering
The study was aimed to prove the possibility to use a tissue engineered
construction based on /33- calcium phosphate (ChronOs™J and stromal cells of
subcutaneous adipose tissue to induce de novo osteogenesis after limited
marginal resection of a mandible branch in Sprague Dawley rats. The technique
of immobilization the given material with progenitor-cells capable not only to
differentiate in the osteogenic way but also to provide the implant with
nutrients via vessel formation was developed. The vessel number was revealed to
increase within the cell-containing implant as well as accelerated formation of
functioning osseous tissue as compared with the controls.
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