cellTranspl.ru - Regenerative regeneration of multiiayered corneal epithelium: biotechnological potential

Regenerative regeneration of multiiayered corneal epithelium: biotechnological potential

PDF, Volume III, №4, 2008

V.G. Gololobov¹, I.V. Gayvoronsky¹, R.V. Deev¹, A.S. Rudko¹, V.N. Ellinidi², N.V. Anikeeva², M.V. Sukhinin¹

1 Kirov Military Medical Academy, Saint Petersburg, Russia
2 Nikiforov Russian Center of Emergency and Radiation Medicine, Saint Petersburg, Russia

Keywords:
epithelium, cornea, damage, regeneration, Ki-67

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The regularities of reparative regeneration of corneal stratified epithelium to be taken into consideration when developing new remedial treatments have been revealed in a rabbit model damage. The epithelium was removed from the entire cornea surface in all the animals. Then the animals were divided into four groups. In the first (control) group the growth zone of the limb was not damaged. In the 2nd group the limb growth zone its was ablated/excised/dissected in one third of its circle, while in the 3rd and 4th groups the limb growth zone was dissected in Ѕ and 2/3 of the limbal circle respectively. The results were evaluated in 6 h, 1, 3, 6 and 15 days with fluorescent staining and visualization of the cornea surface through the slit lamp as well as histologic and immunohistochemical methods. The data received are the basis for developing new biotechnologic therapies for proper regeneration of the cornea surface.

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