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Cultivation properties of human embryonic stem cells in the absence of serum and feeder layer

PDF, Volume III, №3, 2008

E.S. Philonenko, A.N. Kamnev, V.G. Zautorov, M.V. Shutova, L.V. Tskhovrebova, A.N. Bogomazova, S.L. Kiselev, M.A. Lagarkova

Vavilov Institute of General Genetics, Moscow, Russia

Keywords:
human embryonic stem cells (hESCs), cultivation, pluripotency

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Cultivation conditions for five human embryonic stem cell lines (hESM01, hESM02, hESM03, hESM04, HES-9) were switched from s e r u m replacement containing medium with mouse embryonic fibroblasts as a feeder to two commercialy available media (mTESR1 by StemCell Technologies, Canada and StemPro by Invitrogen, USA). Cell lines were maintained in these media for more than 20 passages. Matrigel (BD Biosciences, USA) was used as a substrate. hESCs cultivated in defined media and without animal derived components r e t a i n their pluripotency and differentiation ability. Changes in morphology of feeder-free hESC colonies were observed. Feeder-free hESC cultivation gives possibility to get larger amount of cells due to increase of proliferative activity.

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