cellTranspl.ru - The morphology and chemistry of a rat spinal cord after thoracal segmentectomy and transplantation of polymeric collagen neuromatrix Spherogel E containing incorporated ensheathing neuroepithelial cells

The morphology and chemistry of a rat spinal cord after thoracal segmentectomy and transplantation of polymeric collagen neuromatrix Spherogel E containing incorporated ensheathing neuroepithelial cells

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PDF, Volume III, №2, 2008

I.S. Bryukhovetsky, I.V. Dyuizen, P.A. Motavkin

1. Vladivostok State Medical University, Vladivostok, Russia
2. A.V. Zhirmunsky Institute of Marine Biology, the Far Eastern Branch of the RAS, Vladivostok, Russia

Keywords:
spinal cord, ensheathing neuroepithelial cells, segmentectomy, scar, immunohistochemical study

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Using classical histological methods and immunocytochemical reaction on GAP43 and NF200 we studied the process of tissue reorganizations and neurochimical changes accompanying the scar formation in rat spinal cord after thoracal segmentectomy and subsequent transplantation of olfactory ensheathing cells, incorporated in neuromatrix «Sperogel». In spinal cord of control rats formation on gliomesodermal capsulus was the result of classical histoproliferative reaction. The morphology of scar tissue in experimental rats differed markedly from control group. Its formations began at early stages and were associated with proliferation of both connective tissue and astrocites, and macrophages infiltration. As result the new tissue included a lot of capillaries and bundles of nerve fibers with GAP43 and NF-200 immunopositivity. We conclude that the better tissue repair, their revascularization and nerve regeneration, were the result of two main factors: 1 modulating role of neuromatrix; 2 metabolic and neurotrophic activity of trasplantated olfactory ensheathing cells.

1.Bersenev A.V. Autotransplantaciya obkladochnyh kletok obonyatel'nogo analizatora dlya lecheniya travmy spinnogo mozga - avstralijskoe issledovanie. Kletochnaya transplantologiya i tkanevaya inzheneriya 2005; 1: 13-4.
2. McDonald J.W., Liu X.Z., Qu Y. at al. Transplanted embryonic stem cells survive, differentiate, and promote recovery in injured rat spinal cord. Nat. Med. 1999; 5(12): 1410-2.
3. Motavkin P.A., Pigolkin Ju.I., Kaminskij Ju.V. Gistofiziologiya krovoobraweniya v spinnom mozge. M.: Nauka, 1994.
4. Zyablov V.I. Problemnye voprosy regeneracii nervnoj sistemy. Simferopol', 1986.
5. Stichel C.C., Muller H.M. Experimental strategies to promote axonal regeneration after traumatic central nervous system injury. Prog. Neurobiol. 1998; 56: 119-48
6. Vacanti C.A. Tissue-engineered spinal cord. Transplant. Proc. 2001; 33(1-2): 592-8.
7. Woerly S., Pinet E., De Robertis L. et al. Heterogeneous PHPMA Hydragels forTissue Repair end Axonal Regeneration in the Injured Spinal Cord. J. Biomater. Sci. Polymer Edn. 1998; 9(7): 681-711.
8. Raisman G. A promising therapeutic approach to spinal cord repair. J. R. Soc. Med. 2003; 96(6): 259-61.
9. Bamber N.I., Li H., Aebischer P., Xu X.M. Fetal spinal cord tissue in mini-guidance channels promotes longitudinal axonal growth after grafting into hemisected adult rat spinal cords. Neural Plas. 1999; 6(4): 103-21.
10. Chen X., Fang H., Schwob J. Multipotency of purified, transplanted globose basal cells in olfactory epithelium. J. Сотр. Neurol. 2004; 469(4): 457-74.
11. Brjuhoveckij A.S. Transplantaciya nervnyh kletok i tkanevaya inzheneriya mozga pri nervnyh boleznyah. M.: ZAO "Klinika vosstanovitel'noj intervencionnoj nevrologii i terapii "NejroVita", 2003.
12. Carter L.A., MacDonald J.L., Roskams A.J. et al. Olfactory horizontal basal cells demonstrate a conserved multipotent progenitor phenotype. J. Neurasci. 2004; 24(25): 5670-83.
13. Zhang X., Klueber K.M., Guo Z. et al. Aduilt human olfactory neural progenitors cultured in defined media. Exp. Neural. 2004; 186(2): 112-23.
14. Rogoznjuk Ju.D. Morfologiya spinnomozgovogo rubca pri razlichnyh sposobah soedineniya koncov spinnogo mozga posle ego pererezki v jeksperimente. Aftoref. dis... kand. med. nauk. Simferopol' 1983.
15. Yarygin V.N., Banin V.V., Yarygin K.H., Brjuhoveckij A.S. Regeneraciya spinnogo mozga krys posle torakal'noj segmentjektomiii: rost i vosstanovlenie nervnyh provodnikov. Morfologiya 2006; 1(129); 30-43.
16. Richter W.M., Fletcher P.A., Liu J., Tetzlaff W., Roskams A.J. Lamina propria and olfactory bulb ensheathing cells exhibit differential integration and migration and promote differential axon sprouting in the lesioned spinal cord. J. Neurasci. 2005; 25(46): 10700-11.
17. Chen X., Fang H., Schwob J. Multi potency of purified, transplanted globose basal cells in olfactory epithelium. J. Сотр. Neural. 2004; 469(4): 457-74.
18. Bianco J.I., Perry C, Harkin D.G. et al. Neurotrophin 3 Promotes Purification and Proliferation of Olfactory Ensheathing Cells From Human Nose. Glia 2004; 45(2): 111-23.