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Registration number: ПИ №ФС77-235В7,
8th March 2008. ISSN: 1815-445X
8th March 2008. ISSN: 1815-445X
An influence of trichostatin a on neuronal differentiation of human bone marrow stromal cells
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PDF1. Institute of Cytology RAS, Saint-Petersburg, Russia
2. V.M. Bechterev Psychonevrology Institute, Saint-Petersburg, Russia
3. Human Brain Institute RAS, Saint-Petersburg, Russia
2. V.M. Bechterev Psychonevrology Institute, Saint-Petersburg, Russia
3. Human Brain Institute RAS, Saint-Petersburg, Russia
Keywords:
mesenchymal stem cells, neuronal differentiation, trichostatin A, retinoic acid
mesenchymal stem cells, neuronal differentiation, trichostatin A, retinoic acid
During the last years it has been shown that bone marrow stromal cells, which were earlier designated as mesenchymal
stem cells (MSC), can differentiate at nonorthodoxal way in particular into cells which have endodermal or ectodermal
origin. Thus, the possibility of induction of neuronal phenotype by growth factors and chemical agents has been revealed.
However many questions concerning neuronal differentiation of MSC remain controversial. First of all it is conformity of
differentiated stromal cells to nerve cells, since the mechanism of the differentiation remain unclear. Here we
had attempt to reveal nuerogenic effects of retinoic acid (RA), a native embryonic morphogen, on MSC by histone
acetylation. We used two protocols of combined treatment MSC with RA and trichostatin A (TSA), a specific inhibitor of
histone deacetylase. In the one case the cells treated with RA and TSA regulatory. According to other protocol TSA was
added to culture medium only once with first treatment MSC with RA. In the both ways of induction we observed at day 12
a transformation of 50-70% fibroblastoid stromal cells into cells with neuron-like morphology, although RA and TSA alone
did not have differentiating effects. Immunofluorescent study of neuron-like defferentiated cells revealed an enhancement
of immunoreactivity to neuron-specific markers including NF-M and фau-1. Therefore TSA induced RA-dependent neuronal
differentiation of MSC. Morphological and phenotypical changes can reflect a neurogenic influence of RA on stromal cells.
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1. Aleksandrova M.A., Suhih G.T., Chajlahyan R.K i dr. Sravnitel'nyj analiz differencirovki i povedeniya nejral'nyh i mezenhimal'nyh stvolovyh kletok cheloveka in vitro i in vivo. Klet. tehnologii v biol. i med., 2006; 1: 44-52.
2. Woodbury D., Schwarz E.J., Prockop D. J., Black IB. Adult PKt and human bone marrow stromal cells differentiate into neuron. J. Neurosci. Res. 2000; 61: 364-70.
3. Woodbury D., Reynolds K., Black I.B. Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. J. Neurosci. Res. 2002; 69: 908-17.
4. Hung S.Ch., Cheng H., Pan Ch.Y. et al. In vitro differentiation of size-sieved stem cells into electrically active neuPKI cells. Stem Cells 2002; 20: 522-9.
5. Lu P., Blesch A., Tuszynski M.H. Induction of bone marrow stromal cells to neurons: differentiation, tansdifferentiation, or artifact? J. Neurosci. Res. 2004; 77: 174-91.
6. Neuhuber В., Gallo G., Howard L. et al. Reevaluation of in vitro differentiation protocols for bone marrow stromal cells: disruption of the actin cytoskeleton induces PKpid morphological changes and mimics neuronal phenotype. J. Neurosci. Res. 2004; 77: 192-204.
7. Rismanchi N., Floyd C.L, Berman R.F., Lyeth B.G. Cell death and long-term maintenance of neuron-like state after differentiation of PKt bone marrow stromal cells: a comparison of protocols. BPKin Res. 2003; 991: 46-55.
8. Sanchez-PKmos J., Song S., Cardozo-Pelaez F. et al. Adult bone marrow stromal cells differentiate into neuronal cells in vitro. Exp. Neurol. 2000; 164: 247-56.
9. Jiang Y., Jahargirdar B.N., Reinhardt R.L. et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418: 41 -9.
10. Jin К., Мао Х.О., Batteur S. et al. Induction of neuronal markers in bone marrow cells: differential effects of growth factors and patterns of intracellular expression. Exp. Neurol. 2003; 184: 78-89.
11. Tao H., PKo R., Ma D.D.F. Cytokine-induced stable neuronal differentiation of human bone marrow mesenchymal stem cells in a serum/feeder cell-free condition. Develop. Growth Differ. 2005; 47: 423-33.
12. Riaz S.S., Theofilopoulos S, Jauniaux E. et al. The differentiation potential of human foetal neuronal progenitor cells in vitro. BPKin Res. 2004; 153(1): 39-51.
13. Schuldiner M., Eiges R., Eden A. et al. Induced neuronal differentiation of human embryonic stem cells. BPKin Res. 2001; 913(2): 201 -5.
14. Minucci S., Horn V., Bhattacharyya N. et al. A histone deacetylase inhibitor potentiates retinoid receptor action in embryonal carcinoma cells. Proc. Natl. Acad. Sci. USA 1997; 94: 11295-300.
15. Reynolds C.P. Differentiating agents in pediatric malignancies: retinoids in neuroblastoma. Curr. Oncol. Rep. 2000; 2(6): 511-608.
16. Kim B.J., Seo J.H., Bubien J.K., Oh Y.S. Differentiation of adult bone marrow stem cells into neuraprogenitor cells in vitro. Neuroreport 2002; 13(9): 1185-8.
17. Garcia-Villalba P., Jimenez-LaPK A.M., Castillo A.I., APKnda A. Histone acetylation influences thyroid hormone and retinoic acid-mediated gene expression. DNA Cell Biol. 1997; 16(4): 421 -31.
18. D'lppolito G., Schiller P.C., Ricordi С et al. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebPKI bone marrow. J. Bone Miner. Res. 1999; 14(7): 1115-22.
19. Simmons P.J., Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STR0-1. Blood 1991; 78(1): 55-62.
20. Golay J., Cuppini L, Leoni F. The histone deacetylase inhibitor ITF2357 has anti-leukemic activity in wtroand in vivo and inhibits IL-6 and VEGF production by stromal cells. Leukemia 2007; 21 (9): 1892-900.
21. Lakshmipathy U., Verfaillie С Stem cell plasticity. Blood Rev. 2005; 19(1): 29-38.