...
Registration number: ПИ №ФС77-235В7,
8th March 2008. ISSN: 1815-445X
8th March 2008. ISSN: 1815-445X
Effects of xenotransplantation of neural stem progenitor cells isolated from «olfactory epithelium» on the functional activity of the retina after ischemia modeled
HTML
PDF1. Helmholtz Research Institute of Eye Diseases, Rosmedtechnologies, Moscow
2. Biomed Ltd, Moscow
3. Institute of Gene Biology, Ras, Moscow
2. Biomed Ltd, Moscow
3. Institute of Gene Biology, Ras, Moscow
Keywords:
: neural stem cells, neurotransplantation, «olfactory epithelium», ischemia retinae.
: neural stem cells, neurotransplantation, «olfactory epithelium», ischemia retinae.
Neural stem/ progenitor cells (NSPCs) isolated from the olfactory area of human nasal mucosa are considered as one of the most promising sources for autologous material in cellular therapy and tissue engineering.
Xenotransplantation of NSPCs was preformed in rabbits with ischemia retinae caused by laser coagulation of retina vessels of the I-II class. The cells were introduced into suprachoroidal space. Electroretinographic studies were done to evaluate the extent and degree of functional activity changes of the retina and functional reparative effects of transplantation.
According to electroretinography single transplantation of NSPCs promotes the maintenance and recovery of the retinal function in modeled acute ischemia retinae that correlates with the results of ophthalmoscopy done.
Transplantation of neural stem progenitor cells into suprachoroidal space has been shown to be a safe and effective method of introduction of cells in order to treat retinal disorders due to ischemia.
Unfortunetely, only registered users are allowed to leave comments.
1. Savio Т., Schwab M.E. Rat CNS white matter, but not gray matter, is nonpermissive for neuronal cell adhesion and fiber outgrowth. J. Neurosci. 1989; 4:1126-33.
2. Schwab M.E. Structural plasticity of the adult CNS. Negative control by neurite growth inhibitory signals. Int. J. Dev. Neurosci. 1996; 4: 379-85.
3. .Savaskan N.E.,PlaschkeM., Ninnemann O. etal. Myelindoes not influence the choice bechaviour of entorhinal axons but strongly inhibits their outgrowth length in vitro. Eur. J. Neurosci. 1999; 1:316-26.
4. Little C.W., Cox C, Wyatt J. et al. Correlates of photoreceptor rescue by transplantation of human fetal RPE in RCS rat. Exp. Neural. 1998; 1:155-60.
5. Roisen F.J., Klueber K.M., Lu C.L. et al. Human adult olfactory stem cells. Brain Res. 2001; 890:11 -22.
6. Bamett S. C, Riddell J. S. Olfactory ensheathing cells [OECs) and the treatment of CNS injury: advantages and possible caveats. J. Anat. 2004; 204:57-67.
7. Bandtlow C.E., Heumann R., Schwab M.E., Thoenen H. Cellular localization of nerve growth factor synthesis by in situ hybridization. EM BO J. 1987; 6:891 -899.
8. Acheson A., Barker P.A., Alderson R.F. et al. Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: inhibition by antibodies to NGF. Neural. 1991; 7:265-75.
9. Friedman В., Scherer S.S. Rudge J.S. et al. Regulation of ciliary neurotrophic factor expression in myelin-related Schwann cells in vivo. Neural. 1992; 9:295-305.
10. Bunge R.P., Bunge M.B., Eldridge CF. Linkage between axonal ensheathment and basal lamina production by Schwann cells. Ann. Rev. Neurosci. 1986:9:305-28.